Prof. Ido Braslavsky

Curriculum Vitae

Academic Degrees

1998 - Ph.D. in Physics, Technion, Haifa, Israel. "Imaging of The Temperature Fields Around a Dendritic Crystal" under the supervision of Prof. Steve. G. Lipson

1992 - B.A. in Physics, summa cum laude, Technion, Haifa, Israel.

Positions

October 2017 - current, Head of BSc Program in Biochemistry and Food Science, Institute of Biochemistry, Food Science, and Nutrition.

July 2017, National representative of the ISEKI (Integrating Food Science and Engineering Knowledge into the Food Chain)- Food Association, IFA.

October 2014 - June 2018, Director, Inter-Faculty Biotechnology Program, The Hebrew University of Jerusalem, Faculty of Agriculture, Food, and Environment, Rehovot, Israel

June 2014 - ,Associate Professor, The Hebrew University of Jerusalem, Faculty of Agriculture, Food, and Environment, Rehovot, Israel

April 2010 - June 2014, Senior Lecturer, The Hebrew University of Jerusalem, Faculty of Agriculture, Food, and Environment, Rehovot, Israel

January 2013 - ,Adjunct Professor, Department of Physics and AstronomyOhio University, Athens, OH

September 2009 - December 2012, Associate Professor, Department of Physics and AstronomyOhio University, Athens, OH

August 2003 - August 2009, Assistant Professor, Department of Physics and AstronomyOhio University, Athens, OH

2000-2003 - Postdoc at Caltech, Pasadena, California. Applied Physics, Prof. Steve Quake Group. In collaboration with Prof. Henry Lester, Biology, Caltech.

1998-1999 Postdoc at the Weizmann Institute of Science, Rehovot, Israel. Department of Physics of Complex Systems Prof. Joel Stavans Group

Visiting Scientist

2016-2017 Visiting Prof at Stanford University, California, USA

Dec 2007 Physics of Complex SystemsWeizmann Institute of Science, Rehovot, Israel

July 2004 Department of Materials & InterfacesWeizmann Institute of Science, Rehovot, Israel

Consulting and Companies

2019  Co-founder, MicroIce, nanoliter osmometers.

2018   Co-founder, SavorEat, 3D food printing.

2013-2018 Consulting "Melodea" on ice templating of cellulose structures. 

2004-2007: Consulting "Helicos BioSciences", on single-molecule DNA sequencing techniques.

Membership

Biophysical Society

American Physical Society

Cryobiology Society

Israel Biophysics Society

Current projects

Ice Binding Proteins investigation by:

  • Atomic force microscopy
  • Fluorescence microscopy and microfluidics devices
  • Ice nucleation and supercooling preservation

Cryobiology improvements by infrared heating, directional freezing, and IBPs

Heating methods for 3D printing of food

Awards

2012 Krill Prize

2011 Invitation fellowship programs for research in Japan,JSPS

2000-2001 CMB fellowship, Caltech interdisciplinary program funded by Burroughs Wellcome Fund.

2000 Lester Deutsch, Technion-Caltech fellowship.

1999-1998 The Sir Charles Clore Postdoctoral Fellowship at the Weizmann Institute of Science.

1997-1992 Successive Technion graduate school scholarships including excellence scholarship 1992.

1992 Technion Physics' Dean honors

1991 Participation in distinguished students summer in physics, sponsored by the Amos De-Shalit foundation, Weizmann institute of science.

1991 Technion Physics' Dean honors.

1990 Technion President's honors.

Funding

ERC

FP7

ISF

Marie Curie International Reintegration Grant

NSF

Teaching

Unit Operation In Food Tech

Introduction to Food Science - Principles & Processes

Cryobiology

 

Professional Activities

Co-organizing conference

2017 Ice Binding Protein Conference III, Israel

2014 Ice Binding Proteins Conference II, Japan

2011 Ice Binding Proteins Conference I, Canada

Session Chair

2014 IBP Session 4: Biotechnical and commercial applications for IBPs Co-chairs: Ido Braslavsky and Sakae Tsuda

2013 New Processing Methods / Chairs: D.CHUNG & I. Braslavsky, at the EUROFOODCHEM XVII conference, Istanbul, Turkey.

2013 Cryo, The 50th Anniversary of the Society for Cryobiology's Annual Meeting

2011 Biotechnology Use of Ice Binding Proteins, and poster session chair at the 1st International Ice binding Proteins Conference.

2010 PCI, Physics and Chemistry of Ice, Sapporo Japan, Session 9-A2 topic: Ice and biology.

2006 APS March Meeting Session D9: Methods in Nanobiotechnology

Research Interests

Ice Template

Experimental complex systems physics, Biophysics, Applied optics.

Anti-freeze proteins: Hyperactive Ice binding proteins, Investigation with fluorescence microscopy and microfluidic devices.

Ice physics, Electrofreezing, The double pyramid structure of ice, growth-melt asymmetry,

Refractive index of water, Crystal growth.

Temperature controlled Microfluidics

Single molecule detection and manipulation: DNAProtein interactions, Single molecule DNA sequencing,

DNA Oxidation at the single molecule level.

Optics: Interferometric measurements, Tomographic interferometry, Confocal fluorescent microscopy, Optical tweezers, Evanescent illumination, Darkfield microscopy, Wavefront Shaping.

 

List of Publications

News and cover articles

Infrared Light Offers a Cooler Way to Defrost - Scientific American podcast: 

https://www.scientificamerican.com/podcast/episode/infrared-light-offers-a-cooler-way-to-defrost/

Labyrinth ice pattern formation induced by near-infrared irradiation : 

http://advances.sciencemag.org/content/5/3/eaav1598?rss=1  

https://www.nature.com/articles/d41586-019-01005-z  

First author student Shlomit Guy Preis. 

Phys.org How antifreeze proteins make ice crystals grow

Makor Rishon article on 3d Printing of Food by Professors Braslavsky and Shoseyov

Food Printing in the news Oct 2017: NRGJerusalem post

The Atlantic: The Counterintuitive Way That Microbes Survive in Antarctica 

The Economist, Feb 6th 2016   + Economist podcast (first 5 minutes) 

HORIZON: the EU Research & Innovation magazine, October 2014 

National Geographic article (המהדורה הישראלית), July 2013 

Nature Methods cover of PNAS paper, March 2013 

Science Daily, February 2013 

Jerusalem Post on PNAS article, February 2013 

Cover of the Roy Soc Interface, December 7 2012 issue. 

Krill prize 2012 

ERC Starter Grant 2011: Improved Cryopreservation using Ice Binding Proteins, HUJI Newsletter

PNAS 2010 Superheating: Ohio University , ScienceDaily , PhysOrg'In this Issue' section PNAS March 23 2010

NQPI news letter, Fall 2009, Team studies inner workings of anti-freeze proteins, by Emily Hubbell

BBC: Sub-zero proteins transform dessert, by Victoria Gill , Aug 2009

Cold Case : Office of Research Communications, Ohio University, May 2009

EMBO reports DOI: 10.1038/embor.2008.160 (Sep 2008) Philip Hunter, Harnessing Nature's wisdom. Turning to Nature for inspiration and avoiding her follies,

In sequence, Volume 2 - Number 15 | April 8, 2008 : Helicos Publishes M13 Genome Re-Sequencing Data , related article Physics Today, May 2007 , Feb 2008 APS News: "Physics News in 2007" Related article

Perspectives: Research, Scholarship, and Creative Activity at Ohio University: Fending Off Freeze, Proteins take a bite out of frost

APS2007 : Scientific American , Physics update , ScienceDailyOhio University Press release

Ohio University NanoSpintronics and Nanomagnetics web site: "Brain Freeze" , by Anita Martin 

APS 2006, Virtual pressroom, title: "Fluorescence microscopy studies of the antifreeze proteins"

Physics News Update 2003 Related article

 

Articles

53) Temperature Effect on Polymerase Fidelity, Xue Y, Braslavsky I, Quake SR, 2021,  Journal of Biological Chemistry, in Press, 101270, https://www.jbc.org/article/S0021-9258(21)01073-5/fulltext

52) Adherent cell thawing by infrared radiation, Akalabya Bissoyi and Ido Braslavsky, Cryobiology, 2021, https://doi.org/10.1016/j.cryobiol.2021.08.002

51) Heat flux balance description of unidirectional freezing and melting dynamics on a translational temperature gradient stage, Michael Chasnitsky, Victor Yashunsky, Ido Braslavsky, International Journal of Thermal Sciences, 2020, 161, 106734; https://doi.org/10.1016/j.ijthermalsci.2020.106734

50) Ice Nucleation Properties of Ice-binding Proteins from Snow Fleas, Akalabya Bissoyi, Naama Reicher, Michael Chasnitsky, Sivan Arad,Thomas Koop,Yinon Rudich and Ido Braslavsky, Biomolecules 2019, 9(10), 532; https://doi.org/10.3390/biom9100532 (movie)

49) Improvement of sperm cryo-survival of cynomolgus macaque (Macaca fascicularis) by commercial egg-yolk–free freezing medium with type III antifreeze protein, Shengnan Wang, Yanchao Duan, YapingYan, Chen Adar, Ido Braslavsky, Bingbing Chen, Tianzhuang Huang, Shuai Qiu, Xi Li, Briauna Marie Inglis, Weizhi Ji, Wei Si, Animal Reproduction Science, 2019. 210,106177, https://doi.org/10.1016/j.anireprosci.2019.106177

48) Addressing Complex Matrix Interference Improves Multiplex Food Allergen Detection by Targeted LC–MS/MS, Derek Croote, Ido Braslavsky, Stephen R. Quake, Analytical Chemistry, 2019. https://doi.org/10.1021/acs.analchem.9b01388

47) Direct Cryo Writing of Aerogels via 3D Printing of Aligned Cellulose Nanocrystals Inspired by the Plant Cell Wall Doron Kam,Michael Chasnitsky, Chen Nowogrodski ,Ido Braslavsky,Tiffany Abitbol ,Shlomo Magdassi andOded Shoseyov (2019), Colloids Interfaces 2019, 3(2), 46; https://doi.org/10.3390/colloids3020046

46) Ice-binding proteins and the applicability and limitations of the kinetic pinning model, Chasnitsky M, Braslavsky I. (2019) .Phil. Trans. R. Soc. A 377: 20180391.     https://doi.org/10.1098/rsta.2018.0391  

45)  Labyrinth ice pattern formation induced by near-infrared irradiation, Shlomit Guy Preis, Haim Chayet, Adam Katz, Victor Yashunsky, Avigail Kaner, Shimon Ullman, Ido Braslavsky(2019), Science Advances, Vol 5, eaav1598.  DOI: 10.1126/sciadv.aav1598,   
https://www.nature.com/articles/d41586-019-01005-z 
https://twitter.com/sciencemagazine/status1111984066734243840 

44) Contrasting Behavior of Antifreeze Proteins: Ice Growth Inhibitors and Ice Nucleation Promotors, Lukas Eickhoff, Katharina Dreischmeier, Assaf Zipori, Vera Sirotinskaya, Chen Adar, Naama Reicher, Ido Braslavsky, Yinon Rudich, and Thomas Koop,J. Phys. Chem. Lett.2019105966-972, DOI: 10.1021/acs.jpclett.8b03719     Phys.orgScienceDailyEurekAlert!

43) Saturn-shaped ice burst pattern and fast basal binding of an ice-binding protein from an Antarctic bacterial consortium, Aleksei Kaleda, Lotem Haleva, Guy Sarusi, Tova Pinsky, Marco Mangiagalli, Maya Bar-Dolev, Marina Lotti, Marco Nardini, and Ido Braslavsky, Langmuir 2019 35,23, 7337-7346, DOI:10.1021/acs.langmuir.8b01914

42) Falling water ice affinity purification of ice-binding proteins, Chen Adar, Vera Sirotinskaya, Maya Bar Dolev, Tomer Friehmann, Ido Braslavsky, Scientific Reports 23 July 2018 https://doi.org/10.1038/s41598-018-29312-x

41) Protein-Water-Ice Contact Angle,  Karlsson JOM,  Braslavsky I, Elliott JAW .  Langmuir  2019, 35, 23, 7383-7387.  doi: 10.1021/acs.langmuir.8b01276. 

40) Application of Algebraic Topology to Homologous Recombination of DNA Ido Braslavsky and Joel Stavans, iScience, (2018) 4, 64-67, https://doi.org/10.1016/j.isci.2018.05.008

39) Directional freezing for the cryopreservation of adherent mammalian cells on a substrate. Bahari L, Bein A, Yashunsky V, Braslavsky I (2018) PLoS ONE 13(2): e0192265. doi: https://doi.org/10.1371/journal.pone.0192265  

38) Structure of a bacterial ice binding protein with two faces of interaction with ice. Mangiagalli M, Sarusi G, Kaleda A, Bar Dolev M, Nardone V , Vena VF, Braslavsky I, Lotti M, Nardini M . (2018) FEBS J. 285(9):1653-1666. doi: 10.1111/febs.14434

37) Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice, Shuaiqi Guo, Corey A. Stevens, Tyler D. R. Vance, Luuk L. C. Olijve, Laurie A. Graham, Robert L. Campbell, Saeed R. Yazdi, Carlos Escobedo, Maya Bar-Dolev, Victor Yashunsky, Ido Braslavsky, David N. Langelaan, Steven P. Smith, John S. Allingham, Ilja K. Voets and Peter L. Davies, Science Advances 09 Aug 2017:Vol. 3, no. 8, e1701440 DOI: 10.1126/sciadv.1701440 http://advances.sciencemag.org/content/3/8/e1701440

36) Ice-binding proteins—not only for ice growth control, Maya Bar Dolev & Ido Braslavsky (2017) Temperature, 4:2, 112-113, DOI: 10.1080/23328940.2017.1279255 http://www.tandfonline.com/doi/pdf/10.1080/23328940.2017.1279255  

35) Cryo-protective effect of an ice-binding protein derived from Antarctic bacteria Marco Mangiagalli, Maya Bar-Dolev, Pietro Tedesco, Antonino Natalello, Aleksei Kaleda, Stefania Brocca, Donatella de Pascale, Sandra Pucciarelli, Cristina Miceli, Ido Braslavsky, Marina Lottio 2016 FEBS J 284:163-177 http://dx.doi.org/10.1111/febs.13965

34) Microfluidic Cold-Finger Device for the Investigation of Ice-Binding Proteins Lotem Haleva, Yeliz Celik, Maya Bar-Dolev, Natalya Pertaya-Braun, Avigail Kaner, Peter L. Davies, Ido Braslavsky Biophysical Journal, 111(6) 1143–1150 DOI: http://dx.doi.org/10.1016/j.bpj.2016.08.003

33) Putting life on ice: bacteria that bind to frozen water Maya Bar Dolev, Reut Bernheim, Shuaiqi Guo, Peter L. Davies, Ido Braslavsky J. R. Soc. Interface 13: 20160210. DOI: 10.1098/rsif.2016.0210 
PDF   movies   Link to Interface Blog regarding the article.

32) Ice-Binding Proteins and Their Function, Maya Bar Dolev, Ido Braslavsky, and Peter L. Davies, Annu. Rev. Biochem. 2016. 85:515–42 DOI: 10.1146/annurev-biochem-060815-014546

31) The Grand Challenges of Organ Banking: Proceedings from the first global summit on complex tissue cryopreservation Jedediah K.Lewis, John C.Bischof, Ido Braslavsky, Kelvin G.M.Brockbank, Gregory M.Fahy, Barry J.Fuller, Yoed Rabin, Alessandro Tocchio, Erik J.Woods, Brian G.Wowk, Jason P.Acke, Sebastian Giwa, 2016, Cryobiology, 72:169-182, DOI:10.1016/j.cryobiol.2015.12.001

30) Stevens CA, Drori R, Zalis S , Braslavsky I , Davies PL. (2015) Dendrimer-Linked Antifreeze Proteins Have Superior Activity and Thermal Recovery. Bioconjug Chem.  26:1908-15. 

29) "When are antifreeze proteins in solution essential for ice growth inhibition?", Ran Drori , Peter L. Davies , and Ido Braslavsky, Langmuir 31:5805–5811 DOI: 10.1021/acs.langmuir.5b00345

28) “Experimental Correlation between Thermal Hysteresis Activity and the Distance between Antifreeze Proteins on an Ice Surface”, Ran Drori, Peter L. Davies and Ido Braslavsky, RSC Adv., 2015,5, 7848-7853 . DOI: 10.1039/C4RA12638F

27) "Ice-Binding Proteins that Accumulate on Different Ice Crystal Planes Produces Distinct Thermal Hysteresis Dynamics", Drori, R., Celik, Y., Davies, P.L., and Braslavsky, I., 9 July 2014 doi: 10.1098/ rsif.2014.0526 J. R. Soc. Interface 6 September 2014 vol. 11

26) "Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity", Basu, K., Garnham, C.P., Nishimiya, Y., Tsuda, S., Braslavsky, I., Davies, P.L.. Jan 2014. J. Vis. Exp. (83), e51185 PubMed: 24457629

25) "Model of pore formation in a single cell in a flow-through channel with micro-electrodes", Kaner A, Braslavsky I, Rubinsky B., Biomed Microdevices. 16:181–189  https://doi.org/10.1007/s10544-013-9820-6 2013 Oct 23 

24) "Recent experimental results show the irreversible binding of antifreeze proteins to ice surfaces", Yeliz Celik, Ran Drori, Natalya Pertaya-Braun, Maya Bar-Dolev, Peter L. Davies, Ido Braslavsky. Cryobiology and cryotechnology 59(1), 15-21, 2013

23) "Inhibition of ice growth and recrystallization by zirconium acetate and zirconium acetate hydroxide", Ortal Mizrahy, Maya Bar-Dolev, Shlomit Guy and Ido Braslavsky, (2013) Plos One, 8(3): e59540. doi:10.1371/journal.pone.0059540

22) "Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth", Yeliz Celik, Ran Drori, Natalya Pertaya-Braun, Aysun Altan, Tyler Barton, Maya Bar-Dolev, Alex Groisman, Peter L. Davies, and Ido Braslavsky, PNAS. January 22, 2013.110(4)1309-1314.

21) "LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations", Ido Braslavsky, Ran Drori, Journal of Visualized Experiments. JoVE (72), e4189, doi:10.3791/4189 (2013).  

20) "New Insights into Ice Growth and Melting Modifications by Antifreeze Proteins", Maya Bar-Dolev, Yeliz Celik, J.S. Wettlaufer, Peter L. Davies, and Ido Braslavsky, (2012) J. Royal Society Interface, 2012, 9:3249-59. https://doi.org/10.1098/rsif.2012.0388, Cover of the December 7 2012 issue.

19) “Modeling the Influence of Antifreeze Proteins on Three-Dimensional Ice Crystal Melt Shapes using a Geometric Approach” Jun Jie Liu, Yangzong Qin, Maya Bar Dolev, Yeliz Celik, J. S. Wettlaufer, and Ido Braslavsky, (2012) Proceedings of the Royal Society A, 2012, 468(2147):3311-3322. https://doi.org/10.1098/rspa.2011.0720

18) "Antifreeze Protein from Freeze-Tolerant Grass Has a Beta-Roll Fold with an Irregularly Structured Ice-Binding Site", Adam J. Middleton, Christopher B. Marshall, Frédérick Faucher, Maya Bar-Dolev, Ido Braslavsky, Robert L. Campbell, Virginia K. Walker, Peter L. Davies, Journal of Molecular Biology, 2012 416:713–724.

17) "Compound ice-binding site of an antifreeze protein revealed by mutagenesis and fluorescent tagging" Christopher P. Garnham , Aditya Natarajan , Adam J. Middleton , Mike J. Kuiper , Ido Braslavsky , and Peter L. Davies, 2010 , Biochemistry, 49: 9063-9071.

16) "Superheating of ice crystals in antifreeze protein solutions", Yeliz Celik, Laurie A. Graham, Yee-Foong Mok, Maya Bar, Peter L. Davies, and Ido Braslavsky, PNAS, 2010 107(12)5423-5428pdfSupporting information , 'In this Issue' section PNAS March 23 2010Related news item 2014

15) "Structural basis for the superior activity of the large isoform of snow flea antifreeze protein", Mok YF,Lin FH,Graham LA,Celik Y,Braslavsky I,Davies PL., Biochemistry, 2010 49(11)2593-2603

14) "Caught in the act: the lifetime of synaptic intermediates during the search for homology on DNA", Adam Mani, Ido Braslavsky, Rinat Arbel-Goren, and Joel Stavans, Nucleic Acids Research   38:2036–2043,  https://doi.org/10.1093/nar/gkp1177

13) "Universality of Persistence Exponents in Twodimensional Ostwald Ripening", Jordi Soriano, Ido Braslavsky, Di Xu, Oleg Krichevsky and Joel Stavans, PRL, 2009, 103(22)226101.

12) "Direct visualization of spruce budworm antifreeze protein interacting with an ice crystal: basal plane affinity confers hyperactivity" Natalya Pertaya, Christopher B. Marshall, Yeliz Celik, Peter L. Davies, Ido Braslavsky, Biophysical Journal, 2008, 95(1)333-342related news

11) "Interactions of β-helical Antifreeze Protein Mutants with Ice", Maya Bar, Yeliz Celik, Deborah Fass, Ido Braslavsky,  Crystal Growth & Design 2008, 8, 8, 2954-2963 https://doi.org/10.1021/cg800066g 

10) "Single Molecule DNA Sequencing of a Viral Genome", Timothy D. Harris, Phillip R. Buzby, Hazen Babcock, Eric Beer, Jayson Bowers, Ido Braslavsky, Marie Causey, Jennifer Colonell, James DiMeo, J. William Efcavitch, Eldar Giladi, Jaime Gill, John Healy, Mirna Jarosz, Dan Lapen, Keith Moulton, Stephen R. Quake, Kathleen Steinmann, Edward Thayer, Anastasia Tyurina, Rebecca Ward, Howard Weiss, Zheng Xie,Science, April 4, 2008, Vol. 320 pp. 106-109

 

9) "Growth-melt asymmetry in ice crystals under the influence of spruce budworm antifreeze protein", Natalya Pertaya, Yeliz Celik, Carlos L. DiPrinzio, J. S. Wettlaufer, Peter L. Davies, Ido Braslavsky. J. Phys.: Condens. Matter 19 (2007) 412101 https://doi.org/10.1088/0953-8984/19/41/412101

8) "Fluorescence microscopy evidence for quasi-permanent attachment of antifreeze proteins to ice surfaces", Natalya Pertaya, Christopher B. Marshall, Carlos L. DiPrinzio, Larry Wilen, Erik S. Thomson, J. S. Wettlaufer, Peter L. Davies, Ido Braslavsky, Biophysical Journal, 92(2007)3663

7) "Sequence information can be obtained from single DNA molecules" Ido Braslavsky, Benedict Hebert, Emil Kartalov, and Stephen R. Quake, PNAS, 100(2003)3960 https://doi.org/10.1073/pnas.0230489100F1000, Physics News Update.

6) "Objective-Type Dark Field Illumination for Scattering from Microbeads", Ido Braslavsky, Roee Amit, B. M. Jaffar Ali, Opher Gileadi, Amos Oppenheim, and Joel Stavans, Applied Optics, 40 (2001) 5650 https://doi.org/10.1364/AO.40.005650

5) "Compaction of Single DNA Molecules Induced by Binding of Integration Host Factor (IHF)", B. M. Jaffar Ali, Roee Amit, Ido Braslavsky,Amos B.Oppenheim, Opher Gileadi, and Joel Stavans, PNAS 98 (2001) 10658-10663 https://doi.org/10.1073/pnas.181029198

4) "The Double-Pyramid Structure of Dendritic Ice Growing From Supercooled Water" I. Braslavsky and S.G. Lipson, Journal of Crystal Growth 198-199 (1999) 56. https://doi.org/10.1016/S0022-0248(98)00990-7

3) "Imaging fields around growing crystals", by A. G. Notcovich, I. Braslavsky, S. G. Lipson, Journal of Crystal Growth 198-199 (1999) 10.  https://doi.org/10.1016/S0022-0248(98)01233-0

2) "Interferometric Measurement of the Temperature Field in The Vicinity of Ice Crystals Growing From Supercooled Water" by I. Braslavsky and S.G. Lipson, Physica A 249 (1998) 190-195.  https://doi.org/10.1016/S0378-4371(97)00465-2

1) "Electrofreezing Effect and Nucleation of Ice Crystals in Free Growth Experiments" I. Braslavsky and S.G. Lipson, Applied Physics Letter 72 (1998) 264-266.  https://doi.org/10.1063/1.120705

 

Book Chapters: 

B4) "Structure–Function of IBPs and Their Interactions with Ice." Bar-Dolev, M. ; Basu K ; Braslavsky, I. ; Davies P.L.  In Antifreeze Proteins ; Antifreeze Proteins ; Springer, Cham. 2020; Vol. 2. Publisher's Version Abstract

B3) “Freezing and melting hysteresis measurements in solutions of hyperactive antifreeze protein from an Antarctic bacteria”, Celik Y, Drori R, Graham L, Mok YF, Davies PL, Braslavsky I., 2011, Article in proceedings book of Physics and Chemistry of Ice. Edited by Furukawa Y., Sazaki G., Uchida T. and Watanabe N, Hokkaido University Press, Sapporo. arxiv, pdf.

B2) "Single Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis", B. Hebert and I. Braslavsky, in Book titled: "New High Throughput Technologies for DNA Sequencing and Genomics", Edited by Keith Mitchelson, ISBN-13: 978-0-444-52223-8, Elsevier

B1) "Interferometric Tomography Measurement of the Temperature Field in the Vicinity of a Dendritic Crystal Growing from a supercooled Melt" I. Braslavsky and S.G. Lipson, Transactions of Optical methods and data processing in heat and fluid flow, IMECHE, London 1998, p. 423-432. pdf

 

Other Publications: 

O1) After image, Time sequence growth of an ice dendrite, OPN, Optics & Photonics News, May 1998 Vol.9 No.5, p.88.

 

Papers published in arxivs

Particle Ice Front Interaction - The Brownian Ratchet Model Michael Chasnitsky, Victor Yashunsky, Ido Braslavsky, Submitted on 29 Dec 2017, (none peer review physics archive), arXiv:1712.10258 [cond-mat.soft])

Matrix-corrected mass spectrometry enables sensitive detection of food allergens and reveals widespread soy contamination of processed foods, Derek Croote, Ido Braslavsky, Stephen R. Quake, Dec 2017, BioRxiv (This article is a preprint and has not been peer-reviewed), doi: https://doi.org/10.1101/231266

 

Patents

Patent US 7,297,518 B2, "Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension", Stephen Quake, Ido Braslavsky, Benedict Hebert, and Emil Kartalov, Publication date: Nov20, 2007

 

Abstracts

"The dynamic nature of antifreeze protein activity", Ido Braslavsky, Ran Drori, Yeliz Celik, Maya Bar Dolev, Peter L. Davies, Cryobiology 2013 Abstract

"The effect of antifreeze proteins on vitrification–devitrificartion processes in a micro-scale view", Liat Bahari, Ido Braslavsky, Cryobiology 2013 Abstract

"Controlling freezing using infrared radiation", Shlomit Guy, Boris Greenberg, Haim Chayet, Ido Braslavsky, Cryobiology 2013 Abstract

"Inhibition of ice recrystallization by antifreeze proteins", Shiran Zalis, Maya Bar Dolev, Ido Braslavsky, Cryobiology 2013 Abstract

"Ice shaping in solutions of ice-binding proteins – Melting vs growing morphologies", Maya Bar Dolev, J.J. Liu, Yangzong Qin, Yeliz Celik, Ran Drori, John Wettlaufer, Peter L. Davies, Ido Braslavsky, Cryobiology 2013 Abstract

"Kinetics of hyperactive and moderate antifreeze proteins", Ran Drori, Yeliz Celik, Peter L. Davies, Ido Braslavsky, Cryobiology 2013 Abstract

"Ice binding proteins and their dynamic interaction with ice", Ido Braslavsky, Ran Drori, Yeliz Celik, Maya Bar Dolev, Peter L. Davies, Cryobiology 2012 Abstract

"Inorganic material, zirconium acetate, displays similar characteristics to antifreeze protein", Ortal Mizrahy, Maya Bar Dolev, Ido Braslavsky, Cryobiology 2012 Abstract

"The case for irreversible binding of ice-binding proteins to ice", Ido Braslavsky, Yeliz Celik, Ran Drori, Maya Bar, Aysun Altan, Peter L. Davies, Cryobiology 2011 Abstract

"Enhancing the activity of hyperactive antifreeze proteins with additives", Ortal Mizrahy, Maya Bar, Ido Braslavsky, Cryobiology 2011 Abstract

"Microfluidic experiments with ice binding proteins: Evidence for irreversible binding", Yeliz Celik, Ran Drori, Natalya Pertaya, Aysun Altan, Maya Bar, Alex Groisman, Peter L. Davies, Ido Braslavsky, Cryobiology 2011 Abstract

"Ice crystal shapes in antifreeze protein solutions", Ido Braslavsky, Maya Bar, Junjie Liu, Yangzhong Qin, Yeliz Celik, Peter L. Davies, J.S. Wettlaufer, Cryobiology 2010 Abstract

"A new look at the concentration dependence of ice-binding proteins", Yeliz Celik, Natalya Pertaya, Junjie Liu, Yangzhong Qin, Di Xu, Peter L. Davies, Ido Braslavsky, Cryobiology 2009 Abstract

"The control of growth and melting of ice crystals by ice binding proteins", Ido Braslavsky, Yeliz Celik, Natalya Pertaya, Peter L. Davies, Cryobiology 2008 Abstract

"Hyperactive ice binding proteins and their interactions with ice crystals: A microfluidic approach", Yeliz Celik, Natalya Pertaya, Christopher P. Garnham, Peter L. Davies, Ido Braslavsky, Cryobiology 2008 Abstract

"Why are hyperactive ice-binding-proteins so active?", Ido Braslavsky, Yeliz Celik, Natalya Pertaya, Young Eun Choi, Maya Bar, Peter L. Davies, APS March meeting 2008 Abstract

"Growth-melt asymmetry in ice crystals under the influence of spruce budworm antifreeze protein",Ido Braslavsky, Natalya Pertaya, Carlos L. DiPrinzio, J. S. Wettlaufer, Peter L. Davies. .Biophysical Society 2007, Abstract

"A study on the interactions of antifreeze proteins with ice crystals using microfluidics", Yeliz Celik, Natalya Pertaya, Larry Wilen, Alex Groissman, J. S. Wettlaufer, Peter L. Davies, Ido Braslavsky. Biophysical Society 2007, Abstract

"Basal Plane Affinity of an Insect Antifreeze Protein", N. Pertaya, S.Y. Gauthier, P.L. Davies, I. Braslavsky , APS March meeting 2007 Abstract

"Fluorescence microscopy studies of ice surface - antifreeze proteins interactions", Pertaya N., Marshall C.B., DiPrinzio C.L., Wilen L., Thomson E., Wettlaufer J.S., Davies P.L.,Braslavsky I., P 109 116-P037, poster in 11th International Conference on the Physics and Chemistry of Ice, PCI 2006, Bremerhaven, Germany, July 23-28, 2006

"Evidence to DNA Oxidation in single molecule experiments", Douglas wylie and Ido Braslavsky, Master ThesisFrontiers in Chemical Biology: Single Molecules, Cambridge, UK, 3/2006.

"Single Molecule DNA sequencing by Cyclic synthesis", Braslavsky, Harris, Buzby, Babcock, Beer,Causey, Colonell, DiMeo, Efcavitch, Gill, Healy, Ickes ,Jarosz, Karsh, Lapen, Steinmann,Ulmer,Weber, Weiss, Xie. Frontiers in Chemical Biology: Single Molecules, Cambridge, UK, 3/2006.

"Antifreeze Protein Binds Irreversibly to Ice", Braslavsky, Pertaya, Di Prinzio, Thomson, Wilen, Wettlaufer, Marshall, and Davies , APS March meeting, 2006

"Fluorescence microscopy studies of the hyperactive antifreeze protein from an insect" Pertaya, Di Prinzio, Thomson, Wilen, Wettlaufer,Marshall, Davies and Braslavsky, APS March meeting, 2006

"Fluorescence and confocal microscopy studies of ice surface-antifreeze protein interactions" Natalya Pertaya, Carlos L. Di Prinzio, Erik Thomson, Larry Wilen, John Wettlaufer, Peter L. Davies, Ido Braslavsky, APS, March meeting 2005.

"Fluorescence microscopy studies of ice surface-antifreeze protein interactions", Natalya Pertaya, Carlos L. Di Prinzio, Erik Thomson, Larry Wilen, John Wettlaufer, Peter L. Davies, Ido Braslavsky, BIOPHYSICAL JOURNAL 88 (1): 81A-82A Part 2 Suppl. S JAN 2005 Abstract, Biophysics 2005

"Single Molecule Measurements of DNA Polymerase Activity:A Step Towards Single Molecule Sequencing", Ido Braslavsky, Emil Kartalov,Benedict Hebert, Stephen R. Quake, Biophysical Journal, 82(2002)507A

"Optical Measurements of the Temperature Field in the Vicinity of Growing Crystal using Interference Tomography", by Henry Fenichel, I. Braslavsky, and S. G. Lipson, Bulletin of the American Physical Society, Vol. 44, No. 1, Part ii, (1999) 1573.

 

Research Interests

The lab

Many organisms are protected from freezing by antifreeze proteins (AFPs), which bind to ice, modify its morphology, and prevent its further growth.  Since the initial discovery of AFPs in fish, the have been found in insects, plants, bacteria and fungi.  These proteins have a wide range of applications in cryomedicine, cryopreservation and frost protection for transgenic plants and vegetables. AFPs also serve as a model for understanding biomineralization, the processes by which proteins help form bones, teeth and shells.  Yet the mechanism of action of different types of antifreeze proteins is incompletely understood.

In Braslavsky’s group, the kinetics of the interaction between AFP and ice is monitored by fluorescence microscopy.  Several types of AFPs labeled with a fluorescent marker have been prepared mainly by our collaborator Peter Davies.  By putting a fluorescent tag on a fish AFP, we were able to directly visualize AFP binding to ice and demonstrate, by lack of recovery after photo-bleaching, that a fish AFP from ocean pout (type III) adheres irreversibly to ice surfaces.  Additionally, we observed fluorescently labeled hyperactive insect antifreeze protein from spruce budworm on ice crystals.  We find that differences between antifreeze protein types are manifested not only by the shape of the ice crystals but also in the way proteins interact with the ice.

We are currently developing devices that can monitor the fluorescently labeled proteins with high sensitivity.  In collaboration with professor Alex Groismann from UCSD, Braslavsky’s group developed microfluidic devices in which the composition of the solution around tiny ice crystals can be changes.  We plan to use these devices soon to further explore the behaviors of the antifreeze proteins and their interaction with ice.  The system of AFPs and ice can be used as a model platform to understand bio-mineralization processes and thus its importance for future nanotechnology applications. 

 ERC project: Improved Cryopreservation using Ice Binding Proteins 

פרופ' עידו ברסלבסקי מהמכון לביוכימיה, מדעי המזון והתזונה בפקולטה לחקלאות, מזון וסביבה ע"ש רוברט ה. סמית, קיבל את המענק בסך 1,500,000 יורו בעבור מחקרו בנושא שימור בקירור על-ידי חלבונים נצמדי קרח. חלבונים נצמדי קרח מונעים את גדילתם של אורגניזמים רבים ובכך מפחיתים את נזקי הקפיאה שלהם. מענק המחקר מהאיחוד האירופאי הוענק לפרופ' ברסלבסקי לצורך מחקר בסיסי של חלבונים נצמדי קרח ושיפור שימור בקור. פרופ' ברסלבסקי מסביר כי שילוב יעיל של חלבונים נצמדי קרח בשימור בקור יכול להביא למהפכה ביישומים בהם נדרשת שליטה בקפיאה כגון שימור רקמות ואיברים בקור, הגנה מפני נזקי קרה בחקלאות ושיפור איכות המזון הקפוא. 


 Marie Curie Reintegration Grant: Freeze Control in Food by Ice Binding Proteins

 

 

ERC project: Improved Cryopreservation using Ice Binding Proteins

http://newsletter.huji.ac.il/article.php?ID=103

 
פרופ' עידו ברסלבסקי מהמכון לביוכימיה, מדעי המזון והתזונה בפקולטה לחקלאות, מזון וסביבה ע"ש רוברט ה. סמית, קיבל את המענק בסך 1,500,000 יורו בעבור מחקרו בנושא שימור בקירור על-ידי חלבונים נצמדי קרח. חלבונים נצמדי קרח מונעים את גדילתם של אורגניזמים רבים ובכך מפחיתים את נזקי הקפיאה שלהם. מענק המחקר מהאיחוד האירופאי הוענק לפרופ' ברסלבסקי לצורך מחקר בסיסי של חלבונים נצמדי קרח ושיפור שימור בקור. פרופ' ברסלבסקי מסביר כי שילוב יעיל של חלבונים נצמדי קרח בשימור בקור יכול להביא למהפכה ביישומים בהם נדרשת שליטה בקפיאה כגון שימור רקמות ואיברים בקור, הגנה מפני נזקי קרה בחקלאות ושיפור איכות המזון הקפוא. 
 

Several organisms have evolved specialized ice binding proteins (IBPs) that prevent their body fluids from freezing (antifreeze proteins, AFPs), inhibit recrystallization of ice in frozen tissues, or initiate freezing at moderate supercooling temperatures (ice nucleating proteins, INPs). These proteins have many potential applications in agriculture, food preservation, cryobiology, and biomedical science. The ubiquitous presence of IBPs in such organisms indicates the power of these molecules to enable survival under cold conditions. Despite this key role in nature, however, IBPs have been effectively exploited in only one cryopreservation application, namely, recrystallization inhibition in ice cream. Several terrestrial organisms, including insects, have developed very active forms of AFPs. These hyperactive AFPs (hypAFPs) have not been utilized significantly thus far in cryopreservation techniques. The gap between the obvious potential of IBPs and their actual applications stems from a lack of knowledge regarding the mechanisms by which IBPs interact with ice surfaces and how these proteins can assist in cryoprotection. I propose to investigate the mechanism by which IBPs inhibit ice crystallization and the use of such proteins for cryopreserving cells, tissues, and organisms. My group has a strong record in the study of the interactions between IBPs and ice using novel methods that we have developed, including fluorescence microscopy techniques combined with cooled microfluidic devices. We will investigate the interactions of AFPs with ice and the use of hypAFPs in cryopreservation procedures. This research will contribute to an understanding of the mechanisms by which IBPs act, and apply the acquired knowledge to cryopreservation. The successful implementation of IBPs in cryopreservation would revolutionize the field of cryobiology, with enormous implications for cryopreservation applications in general and the frozen and chilled food industry in particular.

 

IRG project: Freeze Control in Food by Ice Binding Proteins

Marie Curie Reintegration Grants abstract: 
The freezing processes play a main role in food science. The storage of food in a frozen form has become one of the most common ways to elongate the shelf-time of many food products. Nevertheless, during freezing and thawing, cell walls can be ruptured by the ice crystals or can be separated by extra-cellular ice growth during the recrystalization process. Ice binding proteins (IBP), which include antifreeze proteins, ice nucleating proteins, and recrystalization inhibitors, hold great promise for improvements in food supply and quality through the prevention of frost damage to crops and the enhancement of preservation technology. The interaction of IBPs with ice crystals suggests that these proteins can be use as a means of controlling ice in food in each level of its production and processing. I propose to investigate freeze control in food by ice binding proteins. My background includes a PhD in ice physics and in particular optical investigation of crystal growth, a post-doctorate work in biophysics and biotechnology and academic position in Ohio University in where I investigate IBPs. In my post-doctorate work at Caltech, I developed instrumentation that was the basis for the biotechnology company Helicos Bioscience, which led to the development of the first instrumentation that can sequence DNA at the single molecule level. In the past few years at Ohio University, I have combined the subjects of ice physics and biophysics and have developed methods for investigating antifreeze proteins in novel ways. I have developed unique instrumentation to assess the activity of the proteins, in particular microfluidics and fluorescence microscopy. At the Hebrew University Faculty of Agriculture, Food and Environment I intend to establish a Food Biophysics lab in which I will continue my basic research on the mechanism of freeze control and investigate the potential of IBPs to improve the quality of different food materials upon cooling, freezing and thawing.

 

Lab members

Lab Members

Postdocs and associated researchers:

Dr. Akalabya Bissoyi,  Vatat scholar

PhD Students:

Shlomit Preis
Vera Sirotinskaya
Michael Chasnitsky
Shay Shemesh
Boris Veltman (Co-advised with Dr.  Evgeni Elzov, Volcani)
Daniel Waiger

MSc students:

Tal Shrem
Guy Sarusi

Amirim student: 

Yarden Lin,  Amirim thesis

Research assistants:

Sivan Benbassat
Yogev Naim

Technician:

Svetlana Pen

Former Lab Members:

Tova Pinsky (Master graduate)
Chen Adar  (Master graduate)
Eyal Heber (Research assistant)
Ilya Savchenko  (Master graduate)
Ma Zhuang  (Master graduate)  
Dr. Maya Bar Dolev -   Research Associate and Lady Davis postdoctoral fellowship awardee  
Dr. Avigail Ben-Or (Ph.D. graduate, co-advisor - Prof Boris Rubinsky)  
Dr. Liat Bahari  (Research Associate)  
Dr. Amir Bein  (Postdoctoral fellow)  
Dr. Victor Yashunsky  (Postdoctoral fellow)  
Dr. Krishna Kant Yadav (Postdoctoral fellow)
Adam Katz  (Master graduate)  
Shiran Zalis  (Master graduate)  
Lotem Haleva  (Master graduate)  
Yulia Baron  (Master graduate)  
Hila Cohen  (Master graduate)  
Dr. Ran Drori  (Ph.D. graduate) 
Maya Drori (Administration asistant)  
Reut Berenhaim  (Master graduate)  
Ortal Mizrahi   (former Ph.D. student)     
Dr. Yeliz Celik  (Ph.D. graduate)    
Dr. Liu JJ   (Postdoctoral fellow)  
Dr. Aysun Atlan  (Postdoctoral fellow)  
Dr. Natalya Pertaya  (Postdoctoral fellow)  
Yangzhong Qin   (former master student)    
Di Xu   (former master student)   
Dr. Yang Eun Choi (Master graduate)   
Duoglas Wylie  (Master graduate)  
Paul Ingram   (undergraduate project)  
Barton Tyler (undergraduate project)
Dr Carlos DiPrinzio (Postdoctoral fellow)

 

Events